PrimerPairs Design

It will take time to download ALL EcoGene Primer Pairs records!
You can use search page to download the gene(s) you specify or go to a topice page to download the genes associated with a specified topic.

Step 1: Choose Download Options

Type of gene

protein

RNA

start primer(forward) length: bp

stop primer(reverse) length: bp

Step 2: Choose Cloning or Deletion

cloning

deletion

Options for Cloning

Set offset start offset:    bp

  

Outside

Inside

stop offset:    bp

Outside

Inside

Add-ons: Enter sequence to add to primer 5' ends

no add-ons

Your sequence

start (fwd) primer add-ons

                                                         end spacer

restriction site

stop (rev) primer add-ons  

                                                         end spacer

restriction site

Options for Deletion

Set offset start offset:    bp inside

stop offset:    bp inside

Add-ons: Enter sequence to add to primer 3' ends

Kan primers 20 bps

Cat primers 20 bps

no add-ons

Your sequence

start (fwd) primer add-ons 

stop (rev) primer add-ons  

Step 3: Select fields you want to download:

EG ID

Gene Name

b#

gene length

start primer

start codon

stop codon

stop primer