GenePage for the bcsQ' pseudogene of Escherichia coli K-12

Primary Gene Name: bcsQ'
EcoGene Accession Number: EG12261
K-12 Gene Accession Number: ECK3519
MG1655 Gene Identifier: b3534
Gene Name Mnemonic: Bacterial cellulose synthesis
Alternate Gene Symbols: yhjQ
Description: Pseudogene reconstruction; required for cellulose production
  # bp Upstream # bp Downstream
MW: 27764.61 ---------250 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 3695233
Left Intergenic Region

Name: bcsA_bcsQ

Length: 4 bp overlap

Orientation: Codirectional-

Left_end: 3695233

Right_end: 3695236

Centisome: 79.61

Genomic Address
Minute or Centisome (%) = 79.61
Right End: 3695985
Right Intergenic Region

Name: bcsQ_yhjR

Length: 11 bp gap

Orientation: Codirectional-

Left_end: 3695986

Right_end: 3695996

Centisome: 79.63

The bcsQ' domestication in-frame stop codon pseudogene defect in K-12 strain W3110 has been repaired, restoring cellulose production, colony morphology, biofilm architecture, and the divergent bcs operons' functions to a presumably wildtype status (Serra, 2013). The bcsQ'(yhjQ') stop codon 6 is translated as an X in the reconstructed protein sequence; other E. coli strains have a Leu codon at this position (K.E.R., 2001, unpublished results cited in UniProt P37655). A previously reported filamentous phenotype for a bcsQ(yhjQ) mutant in a K-12 C600 background (which conflicted with its pseudogene status) has been retested in K-12 strains C600 and MG1655 and no phenotype of knocking out the pseudogene was observed (Kim, 2002; Le Quere, 2009). BcsQ(YhjQ) is required for cellulose production in vivo and for localization of cellulose synthase to a cell pole; the function of BcsQ(YhjQ) was determined in a cellulose-producing E. coli strain 1094, which has an intact copy of bcsQ(yhjQ) (Le Quere, 2009). BcsQ(YhjQ) is a member of the ParA/Soj subfamily of the Mrp/MinD family of P-loop ATPases. The presence of an in-frame stop codon mutation in the bcsQ' gene abolishes cellulose production and bcs divergent operon function in E. coli K-12.

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