GenePage for the aceF gene of Escherichia coli K-12

Primary Gene Name: aceF
EcoGene Accession Number: EG10025
K-12 Gene Accession Number: ECK0114
MG1655 Gene Identifier: b0115
Gene Name Mnemonic: Acetate
Alternate Gene Symbols: None
Description: Pyruvate dehydrogenase, dihydrolipoamide acetyltransferase E2; acetate requirement
  # bp Upstream # bp Downstream
Verified Start MW: 66096.07 ---------630 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 125695
Left Intergenic Region

Name: aceE_aceF

Length: 14 bp gap

Orientation: Codirectional+

Left_end: 125681

Right_end: 125694

Centisome: 2.71

Genomic Address
Clockwise
Minute or Centisome (%) = 2.71
Right End: 127587
Right Intergenic Region

Name: aceF_lpd

Length: 324 bp gap

Orientation: Codirectional+

Left_end: 127588

Right_end: 127911

Centisome: 2.75

The crystal structure of AceF (PDB: 4N72) is derived from E. coli O157:H7 but is identical to the K-12 sequence (Wang, 2014). The pyruvate dehydrogenase holoenzyme is AceE(24)+AceF(24)+Lpd(12). HT_Cmplx3_Cyt: AceF+Lpd.

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BamHI EcoRI HindIII