GenePage for the bioF gene of Escherichia coli K-12

Primary Gene Name: bioF
EcoGene Accession Number: EG10121
K-12 Gene Accession Number: ECK0765
MG1655 Gene Identifier: b0776
Gene Name Mnemonic: Biotin
Alternate Gene Symbols: None
Description: 7-keto-8-amino pelargonic acid synthase
  # bp Upstream # bp Downstream
MW: 41594.26 ---------384 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 810381
Left Intergenic Region

Name: bioB_bioF

Length: 4 bp overlap

Orientation: Codirectional+

Left_end: 810381

Right_end: 810384

Centisome: 17.46

Genomic Address
Clockwise
Minute or Centisome (%) = 17.46
Right End: 811535
Right Intergenic Region

Name: bioF_bioC

Length: 14 bp overlap

Orientation: Codirectional+

Left_end: 811522

Right_end: 811535

Centisome: 17.48

The N-terminal methionine of overexpressed BioF is removed, as confirmed by protein sequencing, but the native RBS was not included in the pET cloning, so BioB is omitted from the Verified Set (Alexeev, 1998).

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BamHI EcoRI HindIII