GenePage for the cheY gene of Escherichia coli K-12

Primary Gene Name: cheY
EcoGene Accession Number: EG10150
K-12 Gene Accession Number: ECK1883
MG1655 Gene Identifier: b1882
Gene Name Mnemonic: Chemotaxis
Alternate Gene Symbols: None
Description: Response regulator for chemotactic signal transduction; single-domain response regulator lacking an output domain; CheA is the cognate sensor protein; autoacetylase
  # bp Upstream # bp Downstream
MW: 14097.37 ---------129 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 1967048
Left Intergenic Region

Name: cheZ_cheY

Length: 10 bp gap

Orientation: Codirectional-

Left_end: 1967038

Right_end: 1967047

Centisome: 42.38

Genomic Address
Minute or Centisome (%) = 42.38
Right End: 1967437
Right Intergenic Region

Name: cheY_cheB

Length: 14 bp gap

Orientation: Codirectional-

Left_end: 1967438

Right_end: 1967451

Centisome: 42.39

The proposed mechanism for optimum chemotaxis suggests alteration of side chain signaling state by rotation of Tyr106 side chain; this is presumably modulated by phosphorylation state of the molecule. A mutation resulting in a tyrosine to tryptophan substitution at position 106 results in a hyperactive phenotype, while a double mutation T87I/Y106W resulted in loss of activity. Substitutions at ILE95 affect FliM binding. CheY can be reversibly acetylated at lysine residues 91, 92, 109, 119, 122, and 126 by acetyl-CoA synthase (Acs) in vitro (Barak, 1992; Barak, 2004). CheY can autoacetylate lysines in its C-terminal region in vitro (Barak, 2006). A high level of lysine acetylation of CheY is detected in vivo, and both Acs-dependent, acetate-stimulated acetylation and Acs-independent acetylation occurs (Yan, 2008). In vivo Acs-dependent lysine acetylation of CheY is consistent with the chemotaxis defects observed in Acs mutants and CheY Lys92 mutants (Barak, 2001) Acs-independent acetylation of CheY could be due to autoacetylation or the activity of a second lysine acetyltransferase such as the lysine acetyltransferase Pka(YfiQ) that has been shown to acetylate Acs lysines in vivo in Salmonella. CobB can deacetylate CheY in vitro and in vivo, reducing FliM binding and chemotaxis (Li, 2010). cobB is a mutational phenotypic suppressor of a cheA cheZ double mutation (Li, 2010). Flagellar regulon.

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