GenePage for the clpB gene of Escherichia coli K-12

Primary Gene Name: clpB
EcoGene Accession Number: EG10157
K-12 Gene Accession Number: ECK2590
MG1655 Gene Identifier: b2592
Gene Name Mnemonic: Caseinolytic protease
Alternate Gene Symbols: htpM
Description: Bichaperone with DnaK for protein disaggregation; disaggregase; protein-dependent ATPase; role in de novo protein folding under mild stress conditions
  # bp Upstream # bp Downstream
Verified Start MW: 95585.02 ---------857 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 2731600
Left Intergenic Region

Name: rrsG_clpB

Length: 442 bp gap

Orientation: Codirectional-

Left_end: 2731158

Right_end: 2731599

Centisome: 58.84

Genomic Address
Minute or Centisome (%) = 58.85
Right End: 2734173
Right Intergenic Region

Name: clpB_ryfD

Length: 21 bp overlap

Orientation: Codirectional-

Left_end: 2734153

Right_end: 2734173

Centisome: 58.90

ClpB and DnaK cooperate (with DnaJ and GrpE) in the bichaperone-mediated, ATP-dependent unfolding of protein aggregates (Zolkiewski, 1999; Goloubinoff, 1999). ClpB exists in two isoforms, long ClpB(alpha)[ClpB93] and short ClpB(beta3[ClpB79], which is initiated from an internal GUG start at codon 149; both starts have been verified by N-terminal protein sequencing (Squires, 1991; Pontis, 1991). The dual translation initiation sites for ClpB have been confirmed using site-directed mutagenesis (Park, 1993). The N-terminal domain, present only in the ClpB long form, is required for efficient binding to protein aggregates and fully effective chaperone function (Barnett, 2005; Chow, 2005). Coordinated expression of both long and short forms of ClpB is required for the protection from thermal killing; the ratio of short form to long form ClpB is 0.4 to 0.5 under a variety of stress conditions (Chow, 2005). ClpB is heat shock protein F84.1 (Squires, 1991). ClpB is regulated by RpoH (Kitagawa, 1991). clpB is required for growth below 8C, which is enabled by transgenic Oleispira antarctica Cpn10/60. HT_Cmplx30_Mem: ClpB+DnaK+InfB+RpsA.

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