GenePage for the dnaA gene of Escherichia coli K-12

Primary Gene Name: dnaA
EcoGene Accession Number: EG10235
K-12 Gene Accession Number: ECK3694
MG1655 Gene Identifier: b3702
Gene Name Mnemonic: DNA
Alternate Gene Symbols: hsm-2
Description: DNA synthesis initiator and global transcription regulator; binds DNA at DnaA boxes, binds cardiolipin and other acidic phospholipids, binds ATP
  # bp Upstream # bp Downstream
MW: 52550.81 ---------467 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 3882326
Left Intergenic Region

Name: dnaN_dnaA

Length: 4 bp gap

Orientation: Codirectional-

Left_end: 3882322

Right_end: 3882325

Centisome: 83.64

Genomic Address
Counterclockwise
Minute or Centisome (%) = 83.64
Right End: 3883729
Right Intergenic Region

Name: dnaA_rpmH

Length: 606 bp gap

Orientation: Divergent

Left_end: 3883730

Right_end: 3884335

Centisome: 83.67

DnaA-ATP is the active form; DnaA-ADP is inactive; switching between the two forms modulates initiation activity. Acidic phospholipids release bound ADP, reactivating DnaA by allowing ATP to rebind. Null mutations in seqA, yccV or rpoN suppress dna46(Ts). Increases in DnaA levels reduce Fis oriC primary binding thus allowing DnaA to bind, this may play a role in the dynamics of prereplication complexes. Collapsed replication forks (primarily within 10 map units of oriC) are a result of the level of initiation induced by elevated dnaA expression. Hda-ADP is the monomeric active form for promoting clamp-mediated DNA-ATP hydrolysis (Su'etsugu, 2008). 2/43 DnaA peptide masses from MS analysis confirm this start codon and indicate that the initial Met is removed (Maillet, 2007).

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BamHI EcoRI HindIII