GenePage for the dnaK gene of Escherichia coli K-12

Primary Gene Name: dnaK
EcoGene Accession Number: EG10241
K-12 Gene Accession Number: ECK0014
MG1655 Gene Identifier: b0014
Gene Name Mnemonic: DNA
Alternate Gene Symbols: groPAB; groPC; groPF; grpC; grpF; seg
Description: Hsp70 molecular chaperone, heat-inducible; bichaperone with ClpB for protein disaggregation
  # bp Upstream # bp Downstream
Verified Start MW: 69114.96 ---------638 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 12163
Left Intergenic Region

Name: yaaI_dnaK

Length: 376 bp gap

Orientation: Divergent

Left_end: 11787

Right_end: 12162

Centisome: 0.25

Genomic Address
Minute or Centisome (%) = 0.26
Right End: 14079
Right Intergenic Region

Name: dnaK_dnaJ

Length: 88 bp gap

Orientation: Codirectional+

Left_end: 14080

Right_end: 14167

Centisome: 0.30

ClpB and DnaK cooperate (with DnaJ and GrpE) in the bichaperone-mediated, ATP-dependent unfolding of protein aggregates (Zolkiewski, 1999; Goloubinoff, 1999). DnaK mediates ATP-regulated binding and release of polypeptide substrates. Binds Zn(II). DnaJ is a co-chaperone for DnaK. Role in the initiation of DNA replication. DnaK has secondary amide peptide bond cis-trans isomerase (APIase) activity. A dnaK mutant is inviable above 42C, or below 8C (as enabled by transgenic Oleispira antarctica Cpn10/60). A tig dnaK double deletion is a synthetic lethal combination at 30 degrees C, however, no lethal effects are observed at 23 degrees C; overproduction of GroEL/GroES at 30 degrees C suppresses synthetic lethality. dnaK, hscA, hscC and yegD are paralogs. After four hours of Zn(II) stress, protein levels increased (Easton, 2006). DnaK lysines K70, K245, K246, K304, K359, K502, K528 and K587 can be modified to N6-succinyllysine (Zhang, 2011). DnaK lysines K109, K245, K304, K421, K556 and K577 can be modified to N6-acetyllysine (Yu, 2008; Zhang, 2009). HT_Cmplx22_Cyt: DnaK+GrpE+GyrA+RpsA. HT_Cmplx30_Mem: ClpB+DnaK+InfB+RpsA.

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