GenePage for the fes gene of Escherichia coli K-12

Primary Gene Name: fes
EcoGene Accession Number: EG10299
K-12 Gene Accession Number: ECK0577
MG1655 Gene Identifier: b0585
Gene Name Mnemonic: Iron
Alternate Gene Symbols: None
Description: Enterochelin esterase
  # bp Upstream # bp Downstream
MW: 45651.94 ---------400 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 612737
Left Intergenic Region

Name: fepA_fes

Length: 242 bp gap

Orientation: Divergent

Left_end: 612495

Right_end: 612736

Centisome: 13.20

Genomic Address
Clockwise
Minute or Centisome (%) = 13.20
Right End: 613939
Right Intergenic Region

Name: fes_ybdZ

Length: 2 bp gap

Orientation: Codirectional+

Left_end: 613940

Right_end: 613941

Centisome: 13.23

26 additional amino acids are added at the N-terminus of Fes despite a reported N-terminal amino acid sequence supposedly confirming the previous start; Fes has been removed from the Verified Set since the start site previously identified now seems unlikely (Pettis, 1988). A peptide encoded in the 26 aa N-terminal extension was identified in a shotgun MS experiment, supporting the upstream start site for Fes (Krug, 2013). Although neither of the proposed start sites for Fes have good RBS sites, the downstream ATG is not conserved whereas the upstream start site and amino acid sequence is conserved.

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BamHI EcoRI HindIII