GenePage for the gapA gene of Escherichia coli K-12

Primary Gene Name: gapA
EcoGene Accession Number: EG10367
K-12 Gene Accession Number: ECK1777
MG1655 Gene Identifier: b1779
Gene Name Mnemonic: Glyceraldehyde phosphate dehydrogenase
Alternate Gene Symbols: None
Description: Glyceraldehyde 3-P dehydrogenase A
  # bp Upstream # bp Downstream
Verified Start MW: 35532.49 ---------331 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 1862771
Left Intergenic Region

Name: msrB_gapA

Length: 341 bp gap

Orientation: Divergent

Left_end: 1862430

Right_end: 1862770

Centisome: 40.12

Genomic Address
Clockwise
Minute or Centisome (%) = 40.13
Right End: 1863766
Right Intergenic Region

Name: gapA_yeaD

Length: 83 bp gap

Orientation: Codirectional+

Left_end: 1863767

Right_end: 1863849

Centisome: 40.15

A gapA mutant is not in the Keio collection; gapA mutants were not expected to grow on the medium used to attempt to isolate them; gapA is not an essential gene (Baba, 2006). Similar to eukaryotic GAPDH. Binds TrxA (Kumar, 2004). GapA binds selenium tightly even after denaturation, possibly as a perselenide to the active site Cys150, perhaps indicative of a role in selenium transport (Lacourciere, 2002). The C-terminal lysine K331 of GapA can be modified to either N6-malonyllysine or N6-succinyllysine; 10 additional GapA lysines can be modified to N6-succinyllysine (Zhang, 2011; Peng, 2011). GapA lysines K132, K138, K184, K198, and K249 can be modifed to N6-acetyllysine (Yu, 2008; Zhang, 2009). The msrB-gapAyeaD divergent operon promoter region binds FNR and gapA-yeaD expression is activated by FNR (Federowicz, 2014).

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BamHI EcoRI HindIII