GenePage for the hisH gene of Escherichia coli K-12

Primary Gene Name: hisH
EcoGene Accession Number: EG10450
K-12 Gene Accession Number: ECK2018
MG1655 Gene Identifier: b2023
Gene Name Mnemonic: Histidine
Alternate Gene Symbols: None
Description: Imidazole glycerol phosphate (IGP) synthase, amidotransferase
  # bp Upstream # bp Downstream
Verified Start MW: 21652.86 ---------196 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 2094535
Left Intergenic Region

Name: hisB_hisH

Length: 1 bp overlap

Orientation: Codirectional+

Left_end: 2094535

Right_end: 2094535

Centisome: 45.12

Genomic Address
Clockwise
Minute or Centisome (%) = 45.12
Right End: 2095125
Right Intergenic Region

Name: hisH_hisA

Length: 1 bp overlap

Orientation: Codirectional+

Left_end: 2095125

Right_end: 2095125

Centisome: 45.14

The N-terminus of purified HisH was sequenced confirming that the fMet is not clipped, but the natural RBS was not used so this additional reference is not cited in the Verified Set (Klem, 1993). In addition to the Edman degradation results noted in the Verifed Set, 1/47 peptides identified by MALDI-TOF-MS and the N-terminus was also sequenced by MS/MS analysis, all confirming that the initial Met is not cleaved (Maillet, 2007).

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BamHI EcoRI HindIII