GenePage for the htpX gene of Escherichia coli K-12

Primary Gene Name: htpX
EcoGene Accession Number: EG10462
K-12 Gene Accession Number: ECK1828
MG1655 Gene Identifier: b1829
Gene Name Mnemonic: High-temperature production
Alternate Gene Symbols: None
Description: Zinc metalloprotease HtpX, membrane-bound; cleaves SecY in vivo and in vitro; self-cleaves; M48 family
  # bp Upstream # bp Downstream
MW: 31923.31 ---------293 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 1911695
Left Intergenic Region

Name: yebQ_htpX

Length: 45 bp gap

Orientation: Convergent

Left_end: 1911650

Right_end: 1911694

Centisome: 41.18

Genomic Address
Counterclockwise
Minute or Centisome (%) = 41.19
Right End: 1912576
Right Intergenic Region

Name: htpX_prc

Length: 191 bp gap

Orientation: Codirectional-

Left_end: 1912577

Right_end: 1912767

Centisome: 41.20

Overexpression of truncated HtpX promotes degradation of incomplete proteins in puromycin-treated cells. HtpX collaborates with FtsH to eliminate abnormal membrane proteins: an hflX ftsH double mutant is temperature-sensitive for growth. Inactivation of putative protease active site residues abolishes ability to cross-complement defects in the FtsH protease. Start codon verified by toeprint analysis (Kornitzer, 1991). HtpX has a cytosolic active site. HtpX in vivo cleavage of SecY is seen when both are overproduced. Cpx regulon. RpoH regulon. Synthetic lethal with hflB(ftsH) in fabZ(SfhC) background. HtpX has two TM helices. htpX, loiP(yggG), bepA(yfgC) and ycaL are paralogs.

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BamHI EcoRI HindIII