GenePage for the ribB gene of Escherichia coli K-12

Primary Gene Name: ribB
EcoGene Accession Number: EG10465
K-12 Gene Accession Number: ECK3032
MG1655 Gene Identifier: b3041
Gene Name Mnemonic: Riboflavin
Alternate Gene Symbols: htrP; luxH
Description: 3,4-dihydroxy-2-butanone 4-phosphate synthase; riboflavin biosynthesis; acid-inducible; homodimeric
  # bp Upstream # bp Downstream
Verified Start MW: 23353.47 ---------217 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 3183813
Left Intergenic Region

Name: zupT_ribB

Length: 489 bp gap

Orientation: Convergent

Left_end: 3183324

Right_end: 3183812

Centisome: 68.58

Genomic Address
Counterclockwise
Minute or Centisome (%) = 68.59
Right End: 3184466
Right Intergenic Region

Name: ribB_yqiC

Length: 373 bp gap

Orientation: Divergent

Left_end: 3184467

Right_end: 3184839

Centisome: 68.61

ribB expression is apparently controlled by a flavin mononucleotide (FMN) responsive riboswitch in the 253 nt 5' UTR. This RFN element 5' UTR has been reported in intergenic sRNA searches as sroG (Vogel, 2003). Attenuation of transcription at the FMN-bound riboswitch produces the 149 nt sroG sRNA, ending at 3182592. The experimentally determined RNA start for sroG is the start of the ribB mRNA. sroG is not annotated as a separate gene and the sroG transcript is part of the ribB mRNA. Mutations in the ribB riboswitch resulting in excretion of riboflavin were isolated as genetic suppressors of a rutE mutation allowing growth on pyrimidines at 37C (Kim, 2010).

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BamHI EcoRI HindIII