GenePage for the icd gene of Escherichia coli K-12

Primary Gene Name: icd
EcoGene Accession Number: EG10489
K-12 Gene Accession Number: ECK1122
MG1655 Gene Identifier: b1136
Gene Name Mnemonic: Isocitrate dehydrogenase
Alternate Gene Symbols: icdA; icdE
Description: Isocitrate dehydrogenase, NADP(+)-specific; e14 attachment site; tellurite reductase
  # bp Upstream # bp Downstream
Verified Start MW: 45756.71 ---------416 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 1195123
Left Intergenic Region

Name: rluE_icd

Length: 171 bp gap

Orientation: Divergent

Left_end: 1194952

Right_end: 1195122

Centisome: 25.74

Genomic Address
Clockwise
Minute or Centisome (%) = 25.75
Right End: 1196373
Right Intergenic Region

Name: icd_ymfD

Length: 493 bp gap

Orientation: Convergent

Left_end: 1196374

Right_end: 1196866

Centisome: 25.77

Icd activity is regulated by AceK-mediated phosphorylation/dephosphorylation at Ser113; phosphorylation inactivates Icd by blocking NADP binding (LaPorte, 1984; Borthwick, 1984; Walsh, 1985; McKee, 1989; Hurley, 1989). This icd allele has a C-terminus added during prophage e14 integration at codon 362, introducing 2 amino acid changes in icd. icdC' encodes the original C-terminus of icd. Binds TrxA (Kumar, 2004). Icd lysines K100 and K242 can be modified to N6-succinyllysine (Zhang, 2011). Icd lysines K142, K177, K230, K235, K242, K265 and K378 can be modified to N6-acetyllysine (Yu, 2008; Zhang, 2009). Modification of Icd K100 and K242 to succinyllysine, as well as K230 to acetyllysine in stationary phase, may affect Icd activity since those residues are critical for activity and substate binding (Lee, 1995; Bolduc, 1995; Yu, 2008; Zhang, 2011). Icd has tellurite reductase branch activity and and an icd mutant is tellurite-sensitive (Reinoso, 2013).

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BamHI EcoRI HindIII