GenePage for the ileS gene of Escherichia coli K-12

Primary Gene Name: ileS
EcoGene Accession Number: EG10492
K-12 Gene Accession Number: ECK0027
MG1655 Gene Identifier: b0026
Gene Name Mnemonic: Isoleucine
Alternate Gene Symbols: ilvS
Description: Isoleucine--tRNA ligase
  # bp Upstream # bp Downstream
Verified Start MW: 104296.84 ---------938 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 22391
Left Intergenic Region

Name: ribF_ileS

Length: 42 bp gap

Orientation: Codirectional+

Left_end: 22349

Right_end: 22390

Centisome: 0.48

Genomic Address
Clockwise
Minute or Centisome (%) = 0.48
Right End: 25207
Right Intergenic Region

Name: ileS_lspA

Length: 1 bp overlap

Orientation: Codirectional+

Left_end: 25207

Right_end: 25207

Centisome: 0.54

ileS is an essential gene (Shiba, 1992). The ileS mutants in the Keio collection have been shown to harbor an intact copy of ileS in addition to the deletion allele (Yamamoto, 2009). The entire protein sequence of IleS has been determined using peptide mapping by MALDI mass spectrometry; 9 aa differences were found as compared to the current DNA-derived protein sequence (300-EL>DV-301; 724G>V;738-AKADSV>PKRTVW-743); whereas the minor differences could be strain polymorphisms, the 6 aa difference may indicate two (in-and-out) closely-spaced DNA sequencing frameshift errors (Baouz, 2009).

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BamHI EcoRI HindIII