GenePage for the kdpD gene of Escherichia coli K-12

Primary Gene Name: kdpD
EcoGene Accession Number: EG10516
K-12 Gene Accession Number: ECK0683
MG1655 Gene Identifier: b0695
Gene Name Mnemonic: K+- DePendence
Alternate Gene Symbols: kac
Description: Sensor kinase for the kdp operon; two-component system; has autokinase activity and KdpE-directed phosphotransferase and phosphatase activities; responds to ionic strength, ATP and potassium; dimeric
  # bp Upstream # bp Downstream
MW: 98718.40 ---------894 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 721730
Left Intergenic Region

Name: kdpE_kdpD

Length: 4 bp overlap

Orientation: Codirectional-

Left_end: 721730

Right_end: 721733

Centisome: 15.55

Genomic Address
Minute or Centisome (%) = 15.55
Right End: 724414
Right Intergenic Region

Name: kdpD_kdpC

Length: 8 bp overlap

Orientation: Codirectional-

Left_end: 724407

Right_end: 724414

Centisome: 15.61

ArcA activates ybfA gene transcription and represses the divergently transcribed kdp operon by binding to the same operator site (Federowicz, 2014). Inhibition of KdpD phosphatase activity leads to an accumulation of phospho-KdpE and activation of kdpFABC transcription. KdpD phosphatase activity directed at KdpE was initiially suggested to be inhibited by turgor pressure, but this has been refuted (Hamann, 2008). Dephosphorylated PTS system EIIA(Ntr) protein PtsN binds to and stimulates sensor kinase KdpD activity, increasing the levels of the cognate phosphorylated response regulator KdpE (Lüttmann, 2009). Since high K+ concentrations lower phosphoryated KdpE, it was suggested that high intracellular potassium downregulates the KdpD autophosphoryation activity, resulting in less phosphotransferance to KdpE (Heermann, 2009; Lüttmann, 2009). A salt shock increases ATP concentrations and probably stimulates KdpD phosphatase activity since a deletion of the ATP-binding site of KdpD deregulates its phosphatase activity (Ohwada, 1987; Jung, 1998).

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