GenePage for the lepA gene of Escherichia coli K-12

Primary Gene Name: lepA
EcoGene Accession Number: EG10529
K-12 Gene Accession Number: ECK2567
MG1655 Gene Identifier: b2569
Gene Name Mnemonic: Leader peptidase
Alternate Gene Symbols: None
Description: Back-translocating Elongation Factor EF4, GTPase; required for efficient and accurate protein synthesis
  # bp Upstream # bp Downstream
MW: 66570.27 ---------599 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 2705325
Left Intergenic Region

Name: lepB_lepA

Length: 15 bp gap

Orientation: Codirectional-

Left_end: 2705310

Right_end: 2705324

Centisome: 58.28

Genomic Address
Counterclockwise
Minute or Centisome (%) = 58.28
Right End: 2707124
Right Intergenic Region

Name: lepA_rseC

Length: 197 bp gap

Orientation: Codirectional-

Left_end: 2707125

Right_end: 2707321

Centisome: 58.32

EF4 is stored at the membrane and released into the cytoplasm at high ionic strength or low temperature (Pech, 2011). Although EF4 improves translational accuracy, it can not reverse paromomycin-induced errors (Qin, 2006). EF4 is a widely conserved protein, the first four domains are related to EF-G domains I, II, III, and V (Qin, 2006). EF4 overexpression is toxic (Qin 2006). EF4 (LepA) is in the lepAB operon but has no known role in signal peptidase I (LepB) activity.

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BamHI EcoRI HindIII