GenePage for the polA gene of Escherichia coli K-12

Primary Gene Name: polA
EcoGene Accession Number: EG10746
K-12 Gene Accession Number: ECK3855
MG1655 Gene Identifier: b3863
Gene Name Mnemonic: Polymerase
Alternate Gene Symbols: resA
Description: DNA polymerase I; required for plasmid replication; translesion synthesis; synthetic lethal with ygdG
  # bp Upstream # bp Downstream
MW: 103118.12 ---------928 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 4046966
Left Intergenic Region

Name: yihG_polA

Length: 363 bp gap

Orientation: Divergent

Left_end: 4046603

Right_end: 4046965

Centisome: 87.18

Genomic Address
Clockwise
Minute or Centisome (%) = 87.19
Right End: 4049752
Right Intergenic Region

Name: polA_spf

Length: 146 bp gap

Orientation: Codirectional+

Left_end: 4049753

Right_end: 4049898

Centisome: 87.25

polA is an essential gene (Joyce, 1984). The polA mutants in the Keio collection have been shown to harbor an intact copy of polA in addition to the deletion allele (Yamamoto, 2009). Residues 324-928 of PolA constitute the Klenow fragment.

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BamHI EcoRI HindIII