GenePage for the priB gene of Escherichia coli K-12

Primary Gene Name: priB
EcoGene Accession Number: EG10764
K-12 Gene Accession Number: ECK4197
MG1655 Gene Identifier: b4201
Gene Name Mnemonic: Primosome
Alternate Gene Symbols: None
Description: Primosomal protein n; ssDNA-binding protein
  # bp Upstream # bp Downstream
Verified Start MW: 11442.18 ---------104 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 4425520
Left Intergenic Region

Name: rpsF_priB

Length: 6 bp gap

Orientation: Codirectional+

Left_end: 4425514

Right_end: 4425519

Centisome: 95.34

Genomic Address
Clockwise
Minute or Centisome (%) = 95.34
Right End: 4425834
Right Intergenic Region

Name: priB_rpsR

Length: 4 bp gap

Orientation: Codirectional+

Left_end: 4425835

Right_end: 4425838

Centisome: 95.35

priB is a non-essential gene, but a priB priC double mutant grows poorly, indicating redundant functionality; an unusually careful approach was successfully utilized to construct the priB302 deletion without disturbing the downstream rpsR-rplI genes (Yamamoto, 2009). PriB is structurally similar to Ssb.

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BamHI EcoRI HindIII