GenePage for the recA gene of Escherichia coli K-12

Primary Gene Name: recA
EcoGene Accession Number: EG10823
K-12 Gene Accession Number: ECK2694
MG1655 Gene Identifier: b2699
Gene Name Mnemonic: Recombination
Alternate Gene Symbols: lexB; recH; rnmB; srf; tif; umuB; umuR; zab
Description: Multifunctional DNA recombination and repair protein; ssDNA-dependent ATPase; synaptase; ssDNA and dsDNA binding protein forming filaments; ATP-dependent homologous DNA strand exchanger; recombinase A; LexA autocleavage cofactor
  # bp Upstream # bp Downstream
Verified Start MW: 37973.37 ---------353 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 2822708
Left Intergenic Region

Name: recX_recA

Length: 68 bp gap

Orientation: Codirectional-

Left_end: 2822640

Right_end: 2822707

Centisome: 60.81

Genomic Address
Counterclockwise
Minute or Centisome (%) = 60.81
Right End: 2823769
Right Intergenic Region

Name: recA_pncC

Length: 79 bp gap

Orientation: Codirectional-

Left_end: 2823770

Right_end: 2823848

Centisome: 60.84

The recA gene was initially identified by a mutation confering UV-sensitivity and causing more than a 1000-fold drop in conjugation recombination frequencies (Clark, 1965). RecA protein was initially observed as Protein X, a membrane protein induced after inhibition of cell division and DNA synthesis (Inouye, 1970). Protein X has been demonstrated to be the product of the recA gene (McEntee, 1977; Gudas, 1977; Emmerson, 1977; Little, 1977). Protein X (RecA) is transcriptionally induced by the inhibition of DNA synthesis to comprise 4% of total cell protein, 10% of which is membrane-bound (Gudas, 1976). RecA is an ATP-dependent dsDNA binding protein and is an ATP-independent, SSB-stimulated ssDNA binding protein, however the ssDNA-RecA complex is stabilized by NTPs (Gudas, 1976; McEntee, 1979; McEntee, 1981). The RecA C-terminal dsDNA binding domain contacts dsDNA at conserved residues W290, I298, G301 and K302 (Aihara, 1997). The dsDNA binding site of RecA also binds the displaced ssDNA strand (Mazin, 1998). RecA R244Q and K246N mutants are proficient in ssDNA binding but are defective in dsDNA binding (Kurumizaka, 1999). RecA does not bind to DNA with extensive cruciform structures, except in the presence of SSB. RecA-dependent autocleavage of the LexA repressor also requires ATP and DNA and signals the SOS response to DNA damage by inducing the LexA regulon. RecA-dependent autocleavage of UmuD is required for polV (UmuD'C) lesion bypass. RecA filamentation is inhibited by RecX. RecA-dependent autocleavage of phage repressors mediates prophage induction after exposure to DNA damaging agents such as UV irradiation. Binds TrxA (Kumar, 2004).

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BamHI EcoRI HindIII