GenePage for the rpoD gene of Escherichia coli K-12

Primary Gene Name: rpoD
EcoGene Accession Number: EG10896
K-12 Gene Accession Number: ECK3057
MG1655 Gene Identifier: b3067
Gene Name Mnemonic: RNA polymerase
Alternate Gene Symbols: alt
Description: RNA polymerase subunit, sigma 70, initiates transcription; housekeeping sigma
  # bp Upstream # bp Downstream
Verified Start MW: 70263.28 ---------613 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 3213047
Left Intergenic Region

Name: dnaG_rpoD

Length: 194 bp gap

Orientation: Codirectional+

Left_end: 3212853

Right_end: 3213046

Centisome: 69.22

Genomic Address
Clockwise
Minute or Centisome (%) = 69.22
Right End: 3214888
Right Intergenic Region

Name: rpoD_mug

Length: 78 bp gap

Orientation: Convergent

Left_end: 3214889

Right_end: 3214966

Centisome: 69.26

rpoD is an essential gene (Nakamura, 1983). The rpoD mutants in the Keio collection have been shown to harbor an intact copy of rpoD in addition to the deletion allele (Yamamoto, 2009). The tsaD-rpsUdnaGrpoD divergent operon promoter region binds ArcA and rpsU-dnaG-rpoD operon expression is activated by ArcA (Federowicz, 2014).

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BamHI EcoRI HindIII