GenePage for the rpsG gene of Escherichia coli K-12

Primary Gene Name: rpsG
EcoGene Accession Number: EG10906
K-12 Gene Accession Number: ECK3328
MG1655 Gene Identifier: b3341
Gene Name Mnemonic: Ribosomal protein, small
Alternate Gene Symbols: None
Description: 30S ribosomal subunit protein S7, mutated stop codon
  # bp Upstream # bp Downstream
Verified Start MW: 20019.09 ---------179 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 3473542
Left Intergenic Region

Name: fusA_rpsG

Length: 27 bp gap

Orientation: Codirectional-

Left_end: 3473515

Right_end: 3473541

Centisome: 74.83

Genomic Address
Counterclockwise
Minute or Centisome (%) = 74.83
Right End: 3474081
Right Intergenic Region

Name: rpsG_rpsL

Length: 96 bp gap

Orientation: Codirectional-

Left_end: 3474082

Right_end: 3474177

Centisome: 74.85

The rpsG gene of K-12 has a mutation converting its stop codon to a Leu codon and extending S7 by 23 amino acids (Reinbolt, 1979). The extended rpsG UGA stop codon in K-12 is poorly recognized and an rluD mutation causes a severe growth defect in K-12 due to UGA readthrough and SsrA tagging; the rluD defect is further exacerbated by a prfB mutation also specific to K-12 (Dreyfus, 2011; Schaub, 2011). HT_Cmplx17_Mem: InfC+RpsG.

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BamHI EcoRI HindIII