GenePage for the rpsL gene of Escherichia coli K-12

Primary Gene Name: rpsL
EcoGene Accession Number: EG10911
K-12 Gene Accession Number: ECK3329
MG1655 Gene Identifier: b3342
Gene Name Mnemonic: Ribosomal protein, small
Alternate Gene Symbols: asuB; strA
Description: 30S ribosomal subunit protein S12; RNA chaperone
  # bp Upstream # bp Downstream
Verified Start MW: 13737.06 ---------124 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 3474178
Left Intergenic Region

Name: rpsG_rpsL

Length: 96 bp gap

Orientation: Codirectional-

Left_end: 3474082

Right_end: 3474177

Centisome: 74.85

Genomic Address
Counterclockwise
Minute or Centisome (%) = 74.85
Right End: 3474552
Right Intergenic Region

Name: rpsL_tusB

Length: 125 bp gap

Orientation: Codirectional-

Left_end: 3474553

Right_end: 3474677

Centisome: 74.86

RimO catalyzes the methylthiolation of Asp-89 of ribosomal protein S12 to form 3-methylthioaspartic acid (Kowalak, 1996; Anton, 2008). YcaO is required for the efficient in vivo RimO-dependent beta-methylthiolation of ribosomal protein S12 (RpsL) on Asp-89 (Strader, 2011). Asp-88 of the mature RpsL is Asp-89 of the unmodified protein. RpsL lysine K108 can be modifed to N6-acetyllysine (Zhang, 2009). Mutations in regions 41-45 (except when Lys42 is replaced with Arg), 87-93 and 53 result in streptomycin resistance. A point mutation at basepair 262 from A to G sustains protein synthesis in late stationary phase and increases the stability of the 70S complex in low magnesium concentrations.

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BamHI EcoRI HindIII