GenePage for the tsaD gene of Escherichia coli K-12

Primary Gene Name: tsaD
EcoGene Accession Number: EG11171
K-12 Gene Accession Number: ECK3054
MG1655 Gene Identifier: b3064
Gene Name Mnemonic: Threonylcarbamoyl-6(Six)-Adenosine
Alternate Gene Symbols: gcp; ygjD
Description: tRNA(NNU) t(6)A37 threonylcarbamoyladenosine modification; glycation binding protein
  # bp Upstream # bp Downstream
MW: 36008.40 ---------337 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 3209530
Left Intergenic Region

Name: ttdT_tsaD

Length: 42 bp gap

Orientation: Convergent

Left_end: 3209488

Right_end: 3209529

Centisome: 69.15

Genomic Address
Minute or Centisome (%) = 69.15
Right End: 3210543
Right Intergenic Region

Name: tsaD_rpsU

Length: 237 bp gap

Orientation: Divergent

Left_end: 3210544

Right_end: 3210780

Centisome: 69.17

The modified tRNA nucleotide t(6)A37 is derived from the labile cyclized ct(6)A37 during extraction; the tRNA threonylcarbamoyladenosine dehydratase TcdA catalyzes the cyclization reaction (Miyauchi, 2013). TsaBCDE are necessary and sufficient for tRNA(NNU) t(6)A37 threonylcarbamoyladenosine modification in vitro (Deutsch, 2012). TsaD(YgjD) was proposed to be a threonine-bicarbonate ligase producing N-carbamoylthreonine which was proposed to then be transferred to the N(6) group of tRNA(NNU) A37 by TsaC(YrdC); TsaB(YeaZ) is required for the essential function of TsaD(YjgD) (El Yacoubi, 2011). Depletion of TsaD(YgjD) prevents tRNA(NNU) t(6)A37 threonylcarbamoyladenosine modification (Srinivasan, 2010). tsaD(ygjD) mutants accumulate toxic glycated proteins (Amadori-modified proteins); the accumulation of glycated FtsZ in TsaD(YgjD)-depleted cells may explain the genome maintenance phenotype and the unusual cell shapes of tsaD(ygjD) mutants (Katz, 2010). S. cerevisiae Qri7, involved in genome maintenance, complements a tsaD(ygjD) mutant growth defect in E. coli (Oberto, 2009). Loss of TsaD(YgjD) in E. coli causes small cell size and nucleoid loss (Obero, 2009). Depletion of TsaD(YgjD) causes a proportion of cells to be enlarged, with a peripheral distribution of DNA; the TsaD(YgjD) depletion phenotype is suppressed by multicopy clones of rstA (Handford, 2009). TsaD(YgjD) family proteins have been termed UGMPs (Universal Genome Maintenance Proteins) but this may be a pleiotropic phenotype primarily due to a defect in tRNA(NNU) t(6)A37 modification (Oberto, 2009). (TsaB)YeaZ interacts with TsaE(YjeE) and TsaD(YgjD), preferring TsaD(YgjD); TsB(YeaZ) can proteolyze TsaD(YgjD) in vitro (Handford, 2009). TsaD(YgjD) is a homolog of a reported glycoprotease named Gcp, but TsaD(YgjD) does not have glycoprotease activity against glycophorin A and multicopy Gcp (M.h.) expression does not complement an E. coli TsaD(YgjD) deficiency, indicating that M. hemolytica Gcp and E. coli YgjD are not isofunctional orthologs; Gcp (M.h.) may not partner properly with the E. coli TsaB(YeaZ) protein (Handford, 2009). The identification of M. hemolytica Gcp as a glycoprotease was probably incorrect (Srinivasan, 2010). Since TsaD(YgjD) does not have glycoprotese activity and the homologous M. hemolytica Gcp is also probably not a glycoprotease, gcp was retired as the primary gene name for tsaD(ygjD). The tsaD-rpsUdnaGrpoD divergent operon promoter region binds ArcA (Federowicz, 2014).

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