GenePage for the epmA gene of Escherichia coli K-12

Primary Gene Name: epmA
EcoGene Accession Number: EG11211
K-12 Gene Accession Number: ECK4151
MG1655 Gene Identifier: b4155
Gene Name Mnemonic: EF-P Modification nomenclature
Alternate Gene Symbols: genX; poxA; yjeA
Description: Elongation Factor P Lys34 lysyltransferase; required for EF-P-mediated translation of polyprolines
  # bp Upstream # bp Downstream
MW: 36976.06 ---------325 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 4382643
Left Intergenic Region

Name: frdA_epmA

Length: 324 bp gap

Orientation: Divergent

Left_end: 4382319

Right_end: 4382642

Centisome: 94.41

Genomic Address
Clockwise
Minute or Centisome (%) = 94.42
Right End: 4383620
Right Intergenic Region

Name: epmA_yjeM

Length: 218 bp gap

Orientation: Codirectional+

Left_end: 4383621

Right_end: 4383838

Centisome: 94.44

The pleiotropic phenotypes associated with epmA(poxA) mutations include synthesis of poxB, hypersensitivity to sulfometuron methyl, alpha-keto butyrate, and many amino acid analogs, slow growth rate, and hypersensitivity to many antibiotics and dyes; these phenotypes are likely due to impaired translation of polyproline-containing proteins, a function of EF-P that requires the EpmAB-mediated lysine34 lysylation (Doerfel, 2012; Ude, 2012). EpmA(YjeA) and EpmB(YjeK) are required to modify EF-P Lys34 by lysylation of the epsilon amino group; failure to modify EF-P with lysine results in a slow growth defect; EpmB(YjeK) may convert alpha-lysyl-EF-P to beta-lysyl-EF-P (Yanagisawa, 2010). A third and final EF-P post-translation modification enzyme EpmC(YfcM) is identified as EF-P-Lys34 hydroxylase; EpmC(YfcM) acts after beta-lysinylation of Lys34 by EpmA(YjeA) and EpmB(YjeK); EpmC(YfcM) adds an oxygen atom to the C5 position of Lys34 and does not modify the added beta-lysine; YjeA and YjeK were renamed as EpmA and EpmB, respectively (Peil, 2012). EpmA(YjeA) and EpmB(YjeK) were predicted to be responsible for the modification of EF-P Lys34 with spermidine, but Lys34 is actually modified with another lysine, not spermidine; epmB(yjeK) is in the efp genetic neighborhood in many strains and all genomes that have the conserved Lys34 in EF-P have epmB(yjeK) orthologs; EpmB(YjeK) is a lysine 2,3-aminomutase homolog (but in a different clade) (Bailly, 2010; Yanagisawa, 2010). The epmA(yjeA) pleiotropic phenotypes are not due to a defect in pyruvate oxidase and the apparent regulation of poxB may be a minor role for EpmA(YjeA). OtherPDB(S. typhimurium): 3G1Z.

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BamHI EcoRI HindIII