GenePage for the mazF gene of Escherichia coli K-12

Primary Gene Name: mazF
EcoGene Accession Number: EG11249
K-12 Gene Accession Number: ECK2776
MG1655 Gene Identifier: b2782
Gene Name Mnemonic: Chromosomal homolog of pem
Alternate Gene Symbols: chpA; chpAK
Description: mRNA interferase toxin, antitoxin is MazE; inhibitor of translation by mRNA cleavage
  # bp Upstream # bp Downstream
MW: 12098.04 ---------111 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 2910756
Left Intergenic Region

Name: mazG_mazF

Length: 70 bp gap

Orientation: Codirectional-

Left_end: 2910686

Right_end: 2910755

Centisome: 62.71

Genomic Address
Counterclockwise
Minute or Centisome (%) = 62.71
Right End: 2911091
Right Intergenic Region

Name: mazF_mazE

Length: 1 bp overlap

Orientation: Codirectional-

Left_end: 2911091

Right_end: 2911091

Centisome: 62.72

MazF is a sequence-specific (ACA) endoribonuclease, induced by amino acid starvation, but in a lon-dependent, ppGpp-independent fashion. MazE homodimer binds two homodimers of MazF. MazF inhibitory effects on translation is reversed by tmRNA. MazF-mediated cell death requires the NNWNN pentapeptide (called EFD for Extracellular Death Factor) appears to be derived from the zwf gene (residues 199-203) and ClpXP is implicated in its production; AsnA appears to be responsible for the NNWDN to NNWNN conversion; ygeO was thought to be required to generate the pentapeptide (in MC4100) but subsequent work demonstrated that overproduction of Zwf could suppresss the YgeO' mutant defect, indicating a secondary role for YgeO' (Kolodkin-Gal, 2007; 2008). ygeO' is an apparent pseudogene in MG1655 and in MC4100 as well, so the ygeO' pseudogene is not responsible for the failure of MG1655 to produce or respond to normal levels of ECD. The MazF ortholog of M. xanthus mediates programmed cell death during fruiting body formation (Nariya, 2008).

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BamHI EcoRI HindIII