GenePage for the codA gene of Escherichia coli K-12

Primary Gene Name: codA
EcoGene Accession Number: EG11326
K-12 Gene Accession Number: ECK0334
MG1655 Gene Identifier: b0337
Gene Name Mnemonic: CytOsine Deaminase
Alternate Gene Symbols: None
Description: Cytosine/isoguanine deaminase
  # bp Upstream # bp Downstream
Verified Start MW: 47590.98 ---------427 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 356171
Left Intergenic Region

Name: codB_codA

Length: 11 bp overlap

Orientation: Codirectional+

Left_end: 356171

Right_end: 356181

Centisome: 7.67

Genomic Address
Clockwise
Minute or Centisome (%) = 7.67
Right End: 357454
Right Intergenic Region

Name: codA_cynR

Length: 336 bp gap

Orientation: Convergent

Left_end: 357455

Right_end: 357790

Centisome: 7.70

An isoguanine deaminase activity of E. coli B was first demonstrated in crude extracts (Friedman, 1951). Isoguanine was found to be a substrate for cytosine deaminase CodA, yielding xanthine as product; this identifies the enzyme solely responsible for isoguanine deaminase activity in K-12 60 years after this orphan enzyme activity was first reported in E. coli (Hitchcock, 2011). Isoguanine (2-oxo-adenine) is an oxidation product of adenine in DNA that is mutagenic to the cell but can be salvaged by CodA.

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BamHI EcoRI HindIII