GenePage for the amiB gene of Escherichia coli K-12

Primary Gene Name: amiB
EcoGene Accession Number: EG11363
K-12 Gene Accession Number: ECK4165
MG1655 Gene Identifier: b4169
Gene Name Mnemonic: Amidase
Alternate Gene Symbols: yjeD
Description: N-acetylmuramyl-L-alanine amidase, periplasmic; role in septal cleavage during cell division; overexpression causes lysis, osmotic hypersensitivity; activated by EnvC
  # bp Upstream # bp Downstream
MW: 47985.23 ---------445 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 4396065
Left Intergenic Region

Name: tsaE_amiB

Length: 18 bp gap

Orientation: Codirectional+

Left_end: 4396047

Right_end: 4396064

Centisome: 94.71

Genomic Address
Clockwise
Minute or Centisome (%) = 94.71
Right End: 4397402
Right Intergenic Region

Name: amiB_mutL

Length: 9 bp gap

Orientation: Codirectional+

Left_end: 4397403

Right_end: 4397411

Centisome: 94.74

EnvC activates AmiA- and AmiB-mediated in vitro murein cleavage; cell lysis induced by EnvC overproduction requires expression of amiA or amiB (Uehara, 2010). In amiABC triple mutants, thickened dark bands termed septal peptidoglycan (SP) rings appear at division sites, representing a cell division structure that accumulates when septal synthesis and hydrolysis by the AmiABC amidases are uncoupled (Priyadarshini, 2007). A mutant lacking five murein hydrolases (amiABCmltCD=MHD90) has a delayed lysis phenotype after beta-lactam treatment (Heidrich, 2002). The first 22 aa of AmiB are predicted to be a type I signal peptide.

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BamHI EcoRI HindIII