GenePage for the tadA gene of Escherichia coli K-12

Primary Gene Name: tadA
EcoGene Accession Number: EG11372
K-12 Gene Accession Number: ECK2557
MG1655 Gene Identifier: b2559
Gene Name Mnemonic: tRNA adenosine deaminase
Alternate Gene Symbols: yfhC
Description: A34-tRNA adenosine deaminase; point mutation confers resistance to HokC(Gef)-mediated cell killing; essential gene; homodimeric
  # bp Upstream # bp Downstream
MW: 18717.53 ---------167 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 2697354
Left Intergenic Region

Name: mltF_tadA

Length: 4 bp overlap

Orientation: Convergent

Left_end: 2697354

Right_end: 2697357

Centisome: 58.11

Genomic Address
Counterclockwise
Minute or Centisome (%) = 58.11
Right End: 2697857
Right Intergenic Region

Name: tadA_pgpC

Length: 57 bp gap

Orientation: Codirectional-

Left_end: 2697858

Right_end: 2697914

Centisome: 58.12

Previously reported non-essential nature was mistakenly based on an insertion that removed the last 17 aa, but the truncated enzyme is active. Three possible start sites annotated in various orthologs: -11, +1, and +12; alignment results ambiguous; +1 chosen due to best RBS.

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BamHI EcoRI HindIII