GenePage for the roxA gene of Escherichia coli K-12

Primary Gene Name: roxA
EcoGene Accession Number: EG11430
K-12 Gene Accession Number: ECK1114
MG1655 Gene Identifier: b1128
Gene Name Mnemonic: Ribosomal oxygenase
Alternate Gene Symbols: ycfD
Description: 50S ribosomal protein L16 Arg81 hydroxylase; ROX; 2-oxoglutarate oxygenase
  # bp Upstream # bp Downstream
MW: 42578.92 ---------373 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 1187119
Left Intergenic Region

Name: pepT_roxA

Length: 48 bp gap

Orientation: Convergent

Left_end: 1187071

Right_end: 1187118

Centisome: 25.57

Genomic Address
Counterclockwise
Minute or Centisome (%) = 25.58
Right End: 1188240
Right Intergenic Region

Name: roxA_phoQ

Length: 75 bp gap

Orientation: Codirectional-

Left_end: 1188241

Right_end: 1188315

Centisome: 25.60

RoxA(YcfD) has 2-oxoglutarate oxygenase activity in vitro and in cells functions as a 50S ribosomal protein L16 arginine hydroxylase selective for Arg81; roxA(ycfD) mutants lack L16 hydroxylation and expressing RoxA(YcfD) restores the modification; roxA(ycfD) mutants grow slowly (Ge, 2012). RoxA(YcfD) is a member of the cupin superfamily Pfam PF8007 and the COG family COG2850. ycfD has been renamed as roxA (personal communication, C.J. Schofield and M.L. Coleman). RoxA is comprised of a Cupin_4 domain (Pfam PF08007).

Go to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePage

BamHI EcoRI HindIII