GenePage for the def gene of Escherichia coli K-12

Primary Gene Name: def
EcoGene Accession Number: EG11440
K-12 Gene Accession Number: ECK3273
MG1655 Gene Identifier: b3287
Gene Name Mnemonic: Deformylase
Alternate Gene Symbols: fms
Description: Peptide deformylase; N-formylmethionylaminoacyl-tRNA deformylase; PDF
  # bp Upstream # bp Downstream
Verified Start MW: 19328.42 ---------169 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 3433690
Left Intergenic Region

Name: smf_def

Length: 129 bp gap

Orientation: Divergent

Left_end: 3433561

Right_end: 3433689

Centisome: 73.97

Genomic Address
Clockwise
Minute or Centisome (%) = 73.98
Right End: 3434199
Right Intergenic Region

Name: def_fmt

Length: 14 bp gap

Orientation: Codirectional+

Left_end: 3434200

Right_end: 3434213

Centisome: 73.99

The def gene is essential unless the fmt formylase gene is also inactivated. Fe(II) is the metal co-factor in vivo (Rajagopalan et al., J. Am. Chem. Soc., 119:12418, 1997). Def was originally thought to be a zinc enzyme.

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BamHI EcoRI HindIII