GenePage for the csrA gene of Escherichia coli K-12

Primary Gene Name: csrA
EcoGene Accession Number: EG11447
K-12 Gene Accession Number: ECK2691
MG1655 Gene Identifier: b2696
Gene Name Mnemonic: Carbon storage regulator
Alternate Gene Symbols: zfiA
Description: Global regulator of carbon source metabolism; RNA binding protein
  # bp Upstream # bp Downstream
MW: 6855.89 ---------61 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 2818961
Left Intergenic Region

Name: serV_csrA

Length: 315 bp gap

Orientation: Codirectional-

Left_end: 2818646

Right_end: 2818960

Centisome: 60.73

Genomic Address
Counterclockwise
Minute or Centisome (%) = 60.73
Right End: 2819146
Right Intergenic Region

Name: csrA_alaS

Length: 234 bp gap

Orientation: Codirectional-

Left_end: 2819147

Right_end: 2819380

Centisome: 60.74

CsrA-mediated and ppGpp-mediated global controls are intertwined; it is proposed both that CsrA-mediated regulation is relieved during the stringent response and that the CsrA-mediated regulation fine-tunes the stringent response (Edwards, 2011). csrA is a conditionally essential gene; a csrA deletion mutant only grows on rich media if the glgCAP operon is disrupted; a csrA deletion mutant also grows on minimal media with pyruvate as the carbon source; the original csrA::kan insertion allele is interrupted at codon 51 but retains partial activity (Timmermans, 2009). CsrA translationally represses the GGDEF diguanylate cyclase enzymes DgcZ(YdeH) and YcdT, which are needed for normal motility (Jonas, 2008). Recombinant CsrA-His6 was N-terminally sequenced, but this does not Verify since translation start was forced at ATG: Met is not clipped: MLIL start. CsrA also postively regulates FlhCD post-translationally, and hence is required for motility. CsrA exerts reciprocal effects on glycolysis versus gluconeogenesis and glycogen biosynthesis. glgC mRNA-binding activity blocks GlgC translation.

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BamHI EcoRI HindIII