GenePage for the yhdJ gene of Escherichia coli K-12

Primary Gene Name: yhdJ
EcoGene Accession Number: EG11498
K-12 Gene Accession Number: ECK3249
MG1655 Gene Identifier: b3262
Gene Name Mnemonic: Systematic nomenclature
Alternate Gene Symbols: ccrM
Description: DNA adenine methyltransferase, SAM-dependent
  # bp Upstream # bp Downstream
MW: 33397.46 ---------294 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 3411653
Left Intergenic Region

Name: fis_yhdJ

Length: 85 bp gap

Orientation: Codirectional+

Left_end: 3411568

Right_end: 3411652

Centisome: 73.50

Genomic Address
Clockwise
Minute or Centisome (%) = 73.50
Right End: 3412537
Right Intergenic Region

Name: yhdJ_yhdU

Length: 83 bp gap

Orientation: Codirectional+

Left_end: 3412538

Right_end: 3412620

Centisome: 73.52

In vitro DNA adenine methyltransferase activity has been demonstrated for YhdJ; overexpression of YhdJ leads to protection of chromosomal DNA from NsiI digestion, but DNA from wild type cells is not protected (Broadbent (2007). DNA from a DNA methyltransferase deficient strain (dam-, dcm-, EcoK-) does not react with anti-N6-methylA or anti-N4-methylC antibodies when YhdJ is expressed from the pBR322 Tet promoter; the yhdJ-containing pBR322 derivative was not resistant to a variety of restriction (personal communication: R. Vaisvila, S. Stickel and R.J. Roberts, unpublished results, April, 2006). The Kossykh (2004) report describing yhdJ as ccrM, an adenine methyltransferase, has been retracted.

Go to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePage

BamHI EcoRI HindIII