GenePage for the thiP gene of Escherichia coli K-12

Primary Gene Name: thiP
EcoGene Accession Number: EG11573
K-12 Gene Accession Number: ECK0068
MG1655 Gene Identifier: b0067
Gene Name Mnemonic: Thiamine (and thiazole)
Alternate Gene Symbols: sfuB; yabK
Description: Thiamine/thiamine pyrophosphate ABC transporter permease
  # bp Upstream # bp Downstream
MW: 59533.17 ---------536 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 72911
Left Intergenic Region

Name: thiQ_thiP

Length: 17 bp overlap

Orientation: Codirectional-

Left_end: 72911

Right_end: 72927

Centisome: 1.57

Genomic Address
Counterclockwise
Minute or Centisome (%) = 1.57
Right End: 74521
Right Intergenic Region

Name: thiP_thiB

Length: 25 bp overlap

Orientation: Codirectional-

Left_end: 74497

Right_end: 74521

Centisome: 1.60

Thiamine (vitamin B1), thiamine pyrophosphate, and thiamine monophosphate uptake is energy-dependent; the uptake of thiamine and thiamine pyrophosphate have been assayed directly (Kawasaki, 1969; Nishimune, 1971; Leach, 1979; Nishimune, 1980; Hollenbach, 2002b). Salmonella thiamine and thiamine pyrophosphate uptake has been assayed directly and is dependent on the thiBPQ operon (Webb, 1998). Although thiamine monophosphate uptake has not been assayed directly, thiamine monophosphate import by ThiBPQ may occur since the ThiB periplasmic binding protein binds thiamine, thiamine monophosphate, and thiamine pyrophosphate (Hollenbach, 2002a). Thiamine, thiamine monophosphate and thiamine pyrophosphate can feed a thiamine auxotroph, indicating all three compounds can be imported (Nakayama, 1972). Biosynthesized thiamine monophosphate is converted to the cofactor thiamine pyrophosphate by thiamine monophosphate kinase (ThiL); imported thiamine is converted to thiamine monophosphate by the salvage enzyme thiamine kinase (ThiK) (Nakayama, 1972; Melnick, 2004). thiBPQ operon expression is controlled by a thiamine pyrophosphate (TPP) responsive riboswitch in the 128 nt 5' UTRl the THI element 5' UTR has been reported in intergenic sRNA searches as sroA/tpe79; attenuation of transcription at the TPP-bound riboswitch produces the 93 nt sroA sRNA, ending at 75608. The experimentally determined RNA start for sroA is the start of the thiBPQ mRNA (Vogel, 2003). sroA is not annotated as a separate gene and the sroA transcript is part of the thiBPQ mRNA. The thiB riboswitch is the target for the antibiotic pyrithiamine.

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BamHI EcoRI HindIII