GenePage for the thiG gene of Escherichia coli K-12

Primary Gene Name: thiG
EcoGene Accession Number: EG11589
K-12 Gene Accession Number: ECK3982
MG1655 Gene Identifier: b3991
Gene Name Mnemonic: Thiamine (and thiazole)
Alternate Gene Symbols: thiB
Description: Thiazole synthase; thiamine-thiazole moiety synthesis; complexes with ThiH
  # bp Upstream # bp Downstream
MW: 26896.10 ---------256 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 4191865
Left Intergenic Region

Name: thiH_thiG

Length: 4 bp overlap

Orientation: Codirectional-

Left_end: 4191865

Right_end: 4191868

Centisome: 90.31

Genomic Address
Counterclockwise
Minute or Centisome (%) = 90.31
Right End: 4192635
Right Intergenic Region

Name: thiG_thiS

Length: 1 bp gap

Orientation: Codirectional-

Left_end: 4192636

Right_end: 4192636

Centisome: 90.33

The ThiG sequence has been confirmed using MS/MS: the measured mass of the intact protein is 26896.5 indicating that the initial Met is not clipped (Kelleher, 1998). ThiG synthesizes the thiazole phosphate intermediate (4-methyl-5-beta-hydroxyethyl thiazole phosphate) in thiamine biosynthesis utilizing dehyrodglycine (derived from tyrosine by ThiH in the ThiGH complex), DXP (1-deoxyxylulose 5-phosphate), S-adenosylmethionine (SAM/AdoMet) and the sulfur donor ThiFS thiocarboxylate (Kriek, 2007). Aldolase family.

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BamHI EcoRI HindIII