GenePage for the galM gene of Escherichia coli K-12

Primary Gene Name: galM
EcoGene Accession Number: EG11698
K-12 Gene Accession Number: ECK0745
MG1655 Gene Identifier: b0756
Gene Name Mnemonic: Galactose
Alternate Gene Symbols: None
Description: Aldose 1-epimerase, type-1 mutarotase; galactose mutarotase; monomeric
  # bp Upstream # bp Downstream
MW: 38190.46 ---------346 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 787797
Left Intergenic Region

Name: gpmA_galM

Length: 201 bp gap

Orientation: Codirectional-

Left_end: 787596

Right_end: 787796

Centisome: 16.97

Genomic Address
Counterclockwise
Minute or Centisome (%) = 16.97
Right End: 788837
Right Intergenic Region

Name: galM_galK

Length: 7 bp overlap

Orientation: Codirectional-

Left_end: 788831

Right_end: 788837

Centisome: 16.99

GalM substrates include D-galactose, D-glucose, D-xylose, D-fucose, L-arabinose, maltose and lactose. Some GalM activity is found in the periplasm. GalM N-terminal sequence demonstrates the initial Met is not cleaved, however the protein was overproduced using a vector-encoded RBS so it is not in the Verifeid Set (Bouffard, 1994). 8/175 GalM peptide masses from MS analysis confirm this start codon and indicate that the initial Met is not removed (Maillet, 2007).

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BamHI EcoRI HindIII