GenePage for the panD gene of Escherichia coli K-12

Primary Gene Name: panD
EcoGene Accession Number: EG11747
K-12 Gene Accession Number: ECK0130
MG1655 Gene Identifier: b0131
Gene Name Mnemonic: Pantothenate
Alternate Gene Symbols: None
Description: Aspartate 1-decarboxylase; pantothenate biosynthesis; residues 1-24 constitute the beta chain; C-terminal 102 residues constitute the alpha chain
  # bp Upstream # bp Downstream
Verified Start MW: 13833.73 ---------126 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 146314
Left Intergenic Region

Name: yadE_panD

Length: 3 bp gap

Orientation: Convergent

Left_end: 146311

Right_end: 146313

Centisome: 3.15

Genomic Address
Counterclockwise
Minute or Centisome (%) = 3.15
Right End: 146694
Right Intergenic Region

Name: panD_yadD

Length: 273 bp gap

Orientation: Divergent

Left_end: 146695

Right_end: 146967

Centisome: 3.16

Aspartate decarboxylase activity synthesizing beta-alanine was confirmed in K-12 when it was first shown to be PanD (Cronan, 1980). panD mutants have two- to threefold elevated intracellular coenzyme A pools when grown in media high in pantothenate or beta-alanine (Cronan, 1982). panD mutants have reduced intracellular coenzyme A pools when the media is depleted of beta-alanine (Jackowski, 1983). PanD is autocleaved into two subunits; the N-terminal serine of the mature PanD alpha subunit is modified to pyruvic acid and involved in catalysis (van Poelje, 1990; Ramjee, 1997; Albert, 1998; Schmitzberger, 2003). Genetically replacing the modified Ser25 with an alanine inactivates PanD (Kennedy, 2004). Monomeric PanM binds to tetrameric PanD and accelerates PanD self-activation by autocleavage at Ser25 in vivo and in vitro; plasmid overexpression of panD overcomes the pantothentate requirement of panM mutants; PanM(PanZ) was initially characterized in Salmonella and subsequently characterized in E. coli (Stuecker, 2012a; Stuecker, 2012b; Nozaki, 2012).

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BamHI EcoRI HindIII