GenePage for the amtB gene of Escherichia coli K-12

Primary Gene Name: amtB
EcoGene Accession Number: EG11821
K-12 Gene Accession Number: ECK0445
MG1655 Gene Identifier: b0451
Gene Name Mnemonic: Ammonia transport
Alternate Gene Symbols: ybaG
Description: Ammonia gas channel; sequesters GlnK, a negative regulator of AmtB activity, to the inner membrane; bi-directional facilitated diffusion
  # bp Upstream # bp Downstream
Verified Start MW: 44514.69 ---------428 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 472966
Left Intergenic Region

Name: glnK_amtB

Length: 29 bp gap

Orientation: Codirectional+

Left_end: 472937

Right_end: 472965

Centisome: 10.19

Genomic Address
Clockwise
Minute or Centisome (%) = 10.19
Right End: 474252
Right Intergenic Region

Name: amtB_tesB

Length: 48 bp gap

Orientation: Convergent

Left_end: 474253

Right_end: 474300

Centisome: 10.22

AmtB is a trimeric inner membrane protein. Each subunit has 11 TM helices; a predicted N-terminal 12th TM helix is actually a cleaved signal peptide. Ammonium is proposed to bind to the periplasmic face of AmtB and then to be deprotonated to ammonia for diffusion through the AmtB channel, then reprotonated by water to hydrate the ammonia gas to ammonium ion in the cytoplasm. AmtB enables growth on ammonium as sole nitrogen source at low ammonium ion concentrations (Soupene, 1998). The human Rh proteins are AmtB homologs and have been variously proposed to act as ammonia channels or as carbon dioxide gas channels.

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BamHI EcoRI HindIII