GenePage for the trmL gene of Escherichia coli K-12

Primary Gene Name: trmL
EcoGene Accession Number: EG11888
K-12 Gene Accession Number: ECK3596
MG1655 Gene Identifier: b3606
Gene Name Mnemonic: tRNA methyltransferase
Alternate Gene Symbols: yibK
Description: tRNA (Leu) mC34,mU34 2'-O-ribose methyltransferase, SAM-dependent
  # bp Upstream # bp Downstream
MW: 17726.25 ---------157 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 3781215
Left Intergenic Region

Name: lldD_trmL

Length: 197 bp gap

Orientation: Codirectional+

Left_end: 3781018

Right_end: 3781214

Centisome: 81.46

Genomic Address
Clockwise
Minute or Centisome (%) = 81.46
Right End: 3781688
Right Intergenic Region

Name: trmL_cysE

Length: 52 bp gap

Orientation: Convergent

Left_end: 3781689

Right_end: 3781740

Centisome: 81.47

It has been suggested that trmL(yibK) and gltE, a streptomycin-dependent mutation affecting the in vitro activity of the GltX glutamate-tRNA ligase, are allelic because trmL(yibK) is the only ORF between the lld(lct) genes and cysE, citing the 1990 genetic map, but this map localization of the unsequenced gltE gene is only approximate, based on Hfr matings and a 5% transduction linkage to xylE; although trmL is consistent with this mapping data, so are many other uncharacterized ORFs; this suggestion has propagated to various databases; although streptomycin-dependent mutations are predicted to be in essential genes, trmL is not an essential gene and it is unclear how the TrmL leucine tRNA methyltransferase could affect in vitro GltX activity, therefore it seems unlikely that trmL(yibK) and gltE are allelic (Murgola, 1970a; Murgola, 1970b; Sofia, 1994). Although it is not certain that a TS revertant of the streptomycin-dependent phenotype of a gltE mutant is also in the gltE gene, transductional mapping of a gltE revertant TS phenotype is consistent with, and more precise, than the 5% xylE linkage; however the observed 63% linkage of pyrE and gltE is at odds with a 25% linkage predicted for the 34 kb pyrE-trmL interval (Lapointe, 1975; K.E.R., 2013). The cysX gene downstream of cysE is the reverse complement of trmL and is probably not a real gene (Tei, 1990). otherPDB (H. influenzae): 1J85, 1MXI.

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BamHI EcoRI HindIII