GenePage for the nuoC gene of Escherichia coli K-12

Primary Gene Name: nuoC
EcoGene Accession Number: EG12084
K-12 Gene Accession Number: ECK2280
MG1655 Gene Identifier: b2286
Gene Name Mnemonic: NADH:ubiquinone oxidoreductase
Alternate Gene Symbols: nuoCD; nuoD
Description: NADH:ubiquinone oxidoreductase, fused CD subunit, complex I; NADH dehydrogenase I
  # bp Upstream # bp Downstream
Verified Start MW: 68236.11 ---------596 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 2402055
Left Intergenic Region

Name: nuoE_nuoC

Length: 2 bp gap

Orientation: Codirectional-

Left_end: 2402053

Right_end: 2402054

Centisome: 51.75

Genomic Address
Counterclockwise
Minute or Centisome (%) = 51.75
Right End: 2403845
Right Intergenic Region

Name: nuoC_nuoB

Length: 105 bp gap

Orientation: Codirectional-

Left_end: 2403846

Right_end: 2403950

Centisome: 51.79

Leif (1995) find the starting amino acids at codon +4 (MVNN), in conflict with the more recent data of David (2002), which does not include these 4 amino acids and the initial Met. Alignment with Yersinia NuoC supports the second ATG annotated here. The reason for the discrepancy is not known as both used native protein preparations, although the protein band sequenced by Leif et al. was not the correct MW for the revised, fused NuoCD protein sequence and Leif used an E. coli B strain. HT_Cmplx18_Mem: NuoB+NuoC.

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BamHI EcoRI HindIII