GenePage for the ytjC gene of Escherichia coli K-12

Primary Gene Name: ytjC
EcoGene Accession Number: EG12164
K-12 Gene Accession Number: ECK4387
MG1655 Gene Identifier: b4395
Gene Name Mnemonic: Systematic nomenclature
Alternate Gene Symbols: gpmB
Description: Phosphatase, function unknown; multicopy suppressor of serB
  # bp Upstream # bp Downstream
MW: 24065.22 ---------215 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 4633797
Left Intergenic Region

Name: yjjX_ytjC

Length: 51 bp gap

Orientation: Divergent

Left_end: 4633746

Right_end: 4633796

Centisome: 99.83

Genomic Address
Clockwise
Minute or Centisome (%) = 99.83
Right End: 4634444
Right Intergenic Region

Name: ytjC_rob

Length: 4 bp overlap

Orientation: Convergent

Left_end: 4634441

Right_end: 4634444

Centisome: 99.84

ytjC is a multicopy suppressor of a serB mutation lacking phosphoserine phosphatase (Patrick, 2007). Although its natural substrate(s) is unknown, purified YtjC(GpmB) has phosphatase activity against the model substrates para-nitrophenyl phosphate and 6-phospho-benzisoxazole (Khersonsky, 2011). GpmB/PhoE/CobC family proteins (COG0406) are sometimes misannotated as predicted phosphoglycerate mutases instead of predicted phosphatases; B. subtilus PhoE (a broad-specificity phosphatase) and S. typhimurium CobC (ribazole-5'-phosphate phosphatase) are COG0406 members with known activities (O'Toole, 1994; Ridgen, 2001). YjtC(GpmB) is not a phosphoglycerate mutase. YtjC(GpmB) is a member of the histidine phosphatase superfamily, represented by the InterPro superfamily profile IPR013078 and CDD cl11399 (also called the dPGM superfamily) including phosphatases and fructose 2,6-bisphosphatases as well as factor-dependent phosphoglycerate mutases; the histidine phosphatase superfamily is characterized by a conserved histidine that is transiently phosphorylated during the catalytic cycle (Fothergill-Gilmore, 1989; Fothergill-Gilmore, 1990; Jedrzejas, 2000; Johnsen, 2007; Rigden, 2008; Foster, 2010). His9 of YtjC is predicted to be the transient phosphohistidine by homology. C10/130 YtjC(GpmB) peptide masses from MS analysis confirm this start codon and indicate that the initial Met is not removed (Maillet, 2007).

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BamHI EcoRI HindIII