GenePage for the cspE gene of Escherichia coli K-12

Primary Gene Name: cspE
EcoGene Accession Number: EG12179
K-12 Gene Accession Number: ECK0616
MG1655 Gene Identifier: b0623
Gene Name Mnemonic: Cold shock protein
Alternate Gene Symbols: gicA; msmC
Description: Transcription antitermination protein; constitutively-expressed cold shock family protein; negative regulator of cspA transcription; binds and melts RNA; binds ssDNA; dimeric
  # bp Upstream # bp Downstream
Verified Start MW: 7463.45 ---------69 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 657292
Left Intergenic Region

Name: pagP_cspE

Length: 174 bp gap

Orientation: Codirectional+

Left_end: 657118

Right_end: 657291

Centisome: 14.16

Genomic Address
Clockwise
Minute or Centisome (%) = 14.16
Right End: 657501
Right Intergenic Region

Name: cspE_flc

Length: 53 bp gap

Orientation: Convergent

Left_end: 657502

Right_end: 657554

Centisome: 14.17

The cspABEG deletion BX04 is cold-sensitive and forms filaments at 15C (Xia, 2001). CspE is a cold shock protein homolog, but is constitutively expressed at 37C. CspE has transcription antitermination activity (Bae, 2000). CspE affects rpoS and uspA expression. CspE is a negative regulator of cspA transcription. A cspE-flc(crcB) double mutant confers camphor-hypersensitivity and exacerbates the phenotypes of topo IV and MukB mutants, indicating that CspE and Flc(CrcB) may have a (possibly redundant) role in maintaining condensed chromosomes, either direct or indirect, possibly through the regulation of other gene products (Sand, 2003). CspE dimers bind ssDNA containing six or more contiguous dT residues with high affinity; CspE dimers binding two distant chromosome regions possible explains a condensin role for cspE when overexpressed with pagP(crcA) and flc(crcB) to suppress mukB mutations and confer camphor resistance (Hu, 1996; Johnston, 2006). Multicopy expression of cspE alone confers 10-fold camphor-resistance, a cspE-flc(crcB) clone confers 100-fold resistance, and the cspE-pagP-crcB clone confers 1000-fold resistance (single flc(crcB) or pagP clones had no effect); it is not known if the pagP gene product is contributing or if the three gene clone just expresses more flc(crcB) and/or cspE (Sand, 2003). A cspE-pagP-flc(crcB) clone increases plasmid DNA supercoiling and reduces DNA gyrase and topoisomerase IV mutant phenotypes (Sand, 2003). The cspE gene was also identified as a "negative regulator of growth in the cold", named as gicA, and submitted to Genbank (L29054) (Ramanathan Y., Narayanarao A.V.S.S., Mathur M., Mahajan S.K., Thesis (1994), University of Bombay, India). otherPDB (S. typhimurium): 3I2Z.

Go to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePage

BamHI EcoRI HindIII