GenePage for the yhiL' pseudogene of Escherichia coli K-12

Primary Gene Name: yhiL'
EcoGene Accession Number: EG12227
K-12 Gene Accession Number: ECK3475
MG1655 Gene Identifier: b4660, b3490, b3489
Gene Name Mnemonic: Systematic nomenclature
Alternate Gene Symbols: yhiK
Description: Pseudogene reconstruction, DUF4049 family protein; FliA regulated
  # bp Upstream # bp Downstream
MW: 61576.33 ---------535 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 3632852
Left Intergenic Region

Name: yhiJ_yhiL

Length: 261 bp gap

Orientation: Codirectional-

Left_end: 3632591

Right_end: 3632851

Centisome: 78.26

Genomic Address join

The following join statement uses GenBank syntax to describe how the reconstructed frameshifted yhiL pseudogene protein sequence was translated from the DNA:

complement(join(3632852..3633265,3633265..3634458))


Counterclockwise
Minute or Centisome (%) = 78.27
Right End: 3634458
Right Intergenic Region

Name: yhiL_yhiM

Length: 382 bp gap

Orientation: Divergent

Left_end: 3634459

Right_end: 3634840

Centisome: 78.30

An apparent frameshift mutation at codon 399 has been reconstructed with an X, which likely was a Lys(K) codon in the original sequence. An intact version of YhiL is present in E. coli O157:H7 as Z4888. The yhiL gene can be transcribed in vitro with sigma28 (FliA) holoenzyme (Yu, 2006).

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BamHI EcoRI HindIII