GenePage for the yhjH gene of Escherichia coli K-12

Primary Gene Name: yhjH
EcoGene Accession Number: EG12252
K-12 Gene Accession Number: ECK3510
MG1655 Gene Identifier: b3525
Gene Name Mnemonic: Systematic nomenclature
Alternate Gene Symbols: None
Description: Cyclic-di-GMP phosphodiesterase, FlhDC-regulated; impaired swimming; suppresses motility defect of hns mutants in multicopy
  # bp Upstream # bp Downstream
MW: 29601.25 ---------255 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 3678420
Left Intergenic Region

Name: yhjG_yhjH

Length: 69 bp gap

Orientation: Codirectional-

Left_end: 3678351

Right_end: 3678419

Centisome: 79.25

Genomic Address
Counterclockwise
Minute or Centisome (%) = 79.25
Right End: 3679187
Right Intergenic Region

Name: yhjH_kdgK

Length: 231 bp gap

Orientation: Divergent

Left_end: 3679188

Right_end: 3679418

Centisome: 79.26

Overexpressed Salmonella typhimurium YhjH (STM3611) enhances motility, counteracts cellulose biosynthesis and the rdar phenotype, and mediates cyclic-di-GMP degradation (Simm, 2004). YhjH contains a cyclic-di-GMP phosphodiesterase EAL domain. The increased motility of the hns suppressing E. coli yhjH clones is consistent with the model that low cyclic di-GMP favors motility over sessility. FlhDC flagellar regulon. YhjH and YdiV are single domain EAL proteins. yhjH mutants have reduced swimming motility, dependent on ycgR and yegE: a null ycgR mutation fully suppress the yhjH motility defect; a null yegE mutation partially suppresses yhjH; a null yedQ mutation barely suppresses yhjH; a null ydaM mutation does not suppress yhjH (Figure 3A) (Pesavento, 2008). yhjH mutants have reduced swimming motility but normal swarming due to a defect in the frequency of flagellar motor switching (Girgis, 2007). The motility defect of yhjH mutations is suppressed by multicopy yfgF and by mutations in ycgR, fliZ, yfiR, yegE, crfC(yjdA), and yjcZ (Girgis, 2007). YhjH appers to have PDE activity despite having a partially defective EAL domain (Sommerfeldt, 2009).

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BamHI EcoRI HindIII