GenePage for the btuF gene of Escherichia coli K-12

Primary Gene Name: btuF
EcoGene Accession Number: EG12334
K-12 Gene Accession Number: ECK0157
MG1655 Gene Identifier: b0158
Gene Name Mnemonic: B12 uptake
Alternate Gene Symbols: yadT
Description: Vitamin B12 periplasmic binding protein
  # bp Upstream # bp Downstream
Verified Start MW: 29366.84 ---------266 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 177662
Left Intergenic Region

Name: yadS_btuF

Length: 37 bp gap

Orientation: Codirectional-

Left_end: 177625

Right_end: 177661

Centisome: 3.83

Genomic Address
Counterclockwise
Minute or Centisome (%) = 3.83
Right End: 178462
Right Intergenic Region

Name: btuF_mtn

Length: 8 bp overlap

Orientation: Codirectional-

Left_end: 178455

Right_end: 178462

Centisome: 3.84

Uncleaved precursor N-terminus was also sequenced, confirming this start site of translation. about 80% of the precursors had the initial Met cleaved. NESG Target EC1 (http://nesg.org).

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BamHI EcoRI HindIII