GenePage for the iaaA gene of Escherichia coli K-12

Primary Gene Name: iaaA
EcoGene Accession Number: EG12407
K-12 Gene Accession Number: ECK0818
MG1655 Gene Identifier: b0828
Gene Name Mnemonic: Isoaspartyl Aminopeptidase; EcAIII
Alternate Gene Symbols: spt; ybiK
Description: isoAsp aminopeptidase, cleaves isoAsp-X dipeptides; Ntn hydrolase; glutathione utilization; weak L-asparaginase activity in vitro (EcAIII); precursor is cleaved into an alpha and beta subunit; heterotetrameric
  # bp Upstream # bp Downstream
MW: 33393.98 ---------321 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 866568
Left Intergenic Region

Name: moeA_iaaA

Length: 203 bp gap

Orientation: Divergent

Left_end: 866365

Right_end: 866567

Centisome: 18.67

Genomic Address
Clockwise
Minute or Centisome (%) = 18.67
Right End: 867533
Right Intergenic Region

Name: iaaA_gsiA

Length: 14 bp overlap

Orientation: Codirectional+

Left_end: 867520

Right_end: 867533

Centisome: 18.69

IaaA is cleaved before Thr179 to expose an N-terminal nucleophile and to produce two subunits. CysB regulon, sensitive to cysteine repression. Start of Salmonella iaaA is confirmed by N-terminal sequencing of IaaA precursor. Salmonella IaaA has been shown to have isoaspartyl-tripeptidase activity also; E. coli probably also has tripeptidase activity. iaaA is a non-essential gene.

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BamHI EcoRI HindIII