GenePage for the epmB gene of Escherichia coli K-12

Primary Gene Name: epmB
EcoGene Accession Number: EG12473
K-12 Gene Accession Number: ECK4140
MG1655 Gene Identifier: b4146
Gene Name Mnemonic: EF-P Modification
Alternate Gene Symbols: yjeK
Description: Required for lysylation of EF-P Lys34; weak lysine 2,3-aminomutase activity; required for EF-P-mediated translation of polyprolines
  # bp Upstream # bp Downstream
MW: 38749.62 ---------342 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 4374629
Left Intergenic Region

Name: yjeJ_epmB

Length: 394 bp gap

Orientation: Codirectional-

Left_end: 4374235

Right_end: 4374628

Centisome: 94.24

Genomic Address
Counterclockwise
Minute or Centisome (%) = 94.25
Right End: 4375657
Right Intergenic Region

Name: epmB_efp

Length: 41 bp gap

Orientation: Divergent

Left_end: 4375658

Right_end: 4375698

Centisome: 94.27

EpmA(YjeA) and EpmB(YjeK) are required to modify EF-P Lys34 by lysylation of the epsilon amino group; failure to modify EF-P with lysine results in a slow growth defect; EpmB(YjeK) may convert alpha-lysyl-EF-P to beta-lysyl-EF-P (Yanagisawa, 2010). Efficient translation of polyproline-containing proteins is the function of EF-P and requires the EpmAB-mediated lysine34 lysylation (Doerfel, 2012; Ude, 2012). A third and final EF-P post-translation modification enzyme EpmC(YfcM) is identified as EF-P-Lys34 hydroxylase; EpmC(YfcM) acts after beta-lysinylation of Lys34 by EpmA(YjeA) and EpmB(YjeK); EpmC(YfcM) adds an oxygen atom to the C5 position of Lys34 and does not modify the added beta-lysine; YjeA and YjeK were renamed as EpmA and EpmB, respectively (Peil, 2012). EpmA(YjeA) and EpmB(YjeK) were predicted to be responsible for the modification of EF-P Lys34 with spermidine, but Lys34 is actually modified with another lysine, not spermidine; epmB(yjeK) is in the efp genetic neighborhood in many strains and all genomes that have the conserved Lys34 in EF-P have epmB(yjeK) orthologs; EpmB(YjeK) is a lysine 2,3-aminomutase homolog (but in a different clade) (Bailly, 2010; Yanagisawa, 2010). EpmB(YjeK) is a homolog of Clostridium subterminale KamA and has weak lysine 2,3-aminomutase activity, but its specific activity is only 0.1% of that of KamA and produces a slightly different product, indicating this may not be a physiologically relevant activity in E. coli (Behshad, 2006). Radical SAM superfamily.

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BamHI EcoRI HindIII