GenePage for the ridA gene of Escherichia coli K-12

Primary Gene Name: ridA
EcoGene Accession Number: EG12524
K-12 Gene Accession Number: ECK4238
MG1655 Gene Identifier: b4243
Gene Name Mnemonic: Reactive intermediate/imine deaminase
Alternate Gene Symbols: yjgF
Description: Enamine/imine deaminase, reaction intermediate detoxification
  # bp Upstream # bp Downstream
Verified Start MW: 13611.59 ---------128 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 4470527
Left Intergenic Region

Name: mgtA_ridA

Length: 205 bp gap

Orientation: Convergent

Left_end: 4470322

Right_end: 4470526

Centisome: 96.31

Genomic Address
Counterclockwise
Minute or Centisome (%) = 96.31
Right End: 4470913
Right Intergenic Region

Name: ridA_pyrI

Length: 72 bp gap

Orientation: Codirectional-

Left_end: 4470914

Right_end: 4470985

Centisome: 96.32

The Salmonella RidA(YjgF) is an enamine and imine deaminase required for the removal of the two unstable, toxic, tautomeric reactive intermediates, aminocrotonate and iminobutyrate, which are produced during the IlvA-mediated, PLP-dependent threonine dehydratase reaction; producing 2-ketobutyrate and releasing ammonia in a hydrolysis reaction that may require catalysis, due either to the low water environment or immediate toxicity in the cell; IlvA also dehydrates serine to yield pyruvate via analogous enamine/imine intermediates, also removed by RidA (Lambrecht, 2012). The Salmonella RidA(YjgF) is required for full IlvE activity and for the dependence of the alternative pyrimidine biosynthesis (APB) pathway of thiamine biosynthesis upon the oxidative pentose phosphate pathway (Schmitz, 2004). RidA(YjgF), RutC, TdcF, YoaB and YjgH are paralogs and members of a highly conserved RidA(YjgF) family (pfam:PF01042) with diverse mutant phenotypes; the family members form homotrimers, some binding 2-ketoglutarate or related compounds, and are probably all reactive intermediate deaminases. The rat homolog PSP (perchloric acid-soluble protein) has been reported to have endoribonuclease activity, Endoribonuclease L, against ss mRNA, inhibiting translation, but the E. coli homologsd are not RNases. PF01042 family.

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BamHI EcoRI HindIII