GenePage for the mdaB gene of Escherichia coli K-12

Primary Gene Name: mdaB
EcoGene Accession Number: EG12656
K-12 Gene Accession Number: ECK3019
MG1655 Gene Identifier: b3028
Gene Name Mnemonic: Modulator of drug activity
Alternate Gene Symbols: mda66
Description: FMN-dependent NADH-quinone reductase; MdaB and YgiN form an enzymatic quinone reduction-oxidation cycle in vitro; possibly mitigates toxicity of quinols and quinones in vivo; dimeric
  # bp Upstream # bp Downstream
Verified Start MW: 21890.91 ---------193 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 3172530
Left Intergenic Region

Name: ygiZ_mdaB

Length: 318 bp gap

Orientation: Divergent

Left_end: 3172212

Right_end: 3172529

Centisome: 68.34

Genomic Address
Clockwise
Minute or Centisome (%) = 68.35
Right End: 3173111
Right Intergenic Region

Name: mdaB_ygiN

Length: 30 bp gap

Orientation: Codirectional+

Left_end: 3173112

Right_end: 3173141

Centisome: 68.36

MdaB overexpression results in resistance to cytotoxic drugs including adriamycin, etoposide and the tumoricidal agent DMP 840. Induced 25-fold by 2-methylene-4-butyrolactone and 2-methyl-1,4-naphthoquinone (menadione). mdaB is in an operon with ygiN. Reduces menadione to menadiol. May have some nitroreductase activity. Paradoxically also reported to be NADPH-specific.

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BamHI EcoRI HindIII