GenePage for the iscS gene of Escherichia coli K-12

Primary Gene Name: iscS
EcoGene Accession Number: EG12677
K-12 Gene Accession Number: ECK2527
MG1655 Gene Identifier: b2530
Gene Name Mnemonic: Iron-sulfur cluster
Alternate Gene Symbols: nuvC; yfhO; yzzO
Description: Cysteine desulfurase, PLP-dependent; used in synthesis of Fe-S clusters and 4-thiouridine; ThiI transpersulfidase; TusA transpersulfidase; YnjE transpersulfidase; MoaD transpersulfidase; pyridoxal phosphate cofactor linked to Lys206
  # bp Upstream # bp Downstream
Verified Start MW: 45089.50 ---------404 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 2660317
Left Intergenic Region

Name: iscU_iscS

Length: 27 bp gap

Orientation: Codirectional-

Left_end: 2660290

Right_end: 2660316

Centisome: 57.31

Genomic Address
Counterclockwise
Minute or Centisome (%) = 57.31
Right End: 2661531
Right Intergenic Region

Name: iscS_iscR

Length: 111 bp gap

Orientation: Codirectional-

Left_end: 2661532

Right_end: 2661642

Centisome: 57.34

IscS desulfurase from cysteine via a Cys328 persulfide to binding partners as a transpersulfidase with ThiI Cys456 for tRNA s(4)U8 modification and with TusA Cys19 to thiolate tRNA U34. IscS has selenocysteine lyase activity in vitro. Two components Factor A (NuvA, 9.3) and Factor B (NuvB, ~45') were originally shown to be required for tRNA s(4)U8 modification with NuvC being the pyridoxal phosphate requiring factor; despite the mapping discrepancy it seems likely that IscS (57.3') is Factor C (NuvC) and ThiI (9.3') is Factor A (NuvA) (Kambampati, 2000). IscU and IscS form a complex (Agar, 2000; Tokumoto, 2002; Kato, 2002; Prischi, 2010). The IscU-IscS complex is stabilized by a covalent disifide bond between Cys328 of IscS and Cys63 of IscU (Kato, 2002). YnjE, IscS, MoaD and MoeB form a complex in vivo (Dahl, 2011). IscU has been classified as a "complex-orphan" protein that is largely unstructured unless bound to a partner, i.e. IscS (Prischi, 2010). 2/409 peptide masses from MS analysis indicate that a start codon eight codons upstream is utilized, but this conflicts with protein sequencing results (Maillet, 2007). PDB structures 3LVJ, 3LVK, 3LVL and 3LVM are derived from E. coli O157:H7 but are identical to the K-12 IscS sequence.

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BamHI EcoRI HindIII